कैन्सर कोशिकाओं पर होम्योपैथी दवाओं के प्रयोग – Homeopathic drugs Natrum sulphuricum and Carcinosin prevent azo dye-induced hepatocarcinogenesis in mice

पिछ्ले दिनों ICRH पर कैन्सर कोशिकाओं पर होम्योपैथिक दवाओं के प्रयोगों पर दो रिसर्च पेपर पब्लिश हुये । कल्याणी यूनिवर्सिटी के श्री अनीसुर रहमान खुदाबख्श और उनके सहयोगियों का  ’Homeopathic drugs Natrum sulphuricum and Carcinosin prevent azo dye-induced hepatocarcinogenesis in mice’ और आमला कैन्सर रिसर्च सेन्टर , केरल के रामदास कुटन , हरीकुमार और अन्य का शोध पत्र , ’ Inhibition of Chemically Induced Carcinogenesis by Drugs Used in Homeopathic Medicine ’ । इन दोनों शोध पत्रों मे ’ Inhibition of Chemically Induced Carcinogenesis by Drugs Used in Homeopathic Medicine ’ मे लगभग वही तथ्यों को दोहराया गया जो इसके पहले प्रशान्त बैनर्जी कैन्सर रिसर्च फ़ाउन्डेशन ने अपने कई शोध पत्रों मे रुटा और उअसके पर्योगों पर दिये  थे , देखें विस्तृत वर्णन के लिये यहाँ ।

लेकिन बात करते हैं अनीसुर रहमान खुदाबख्श के शोध पत्र की । यह पेपर लीक से हट्कर था । प्रथम तो इसमे होम्योपैथी के मूलभूत सिद्धातों (  एकमैव औषधि- single medicine ) को नजरांदाज किया गया और दूसरा औषधि के परीक्षण के लिये चूहों का सहारा लिया गया । तो सबसे पहले विवरण :

विवरण :

इस शोध  का मकसद होम्योपैथिक औषधि कारसीनोसिन २०० ( carcinocin 200 ) और नैट्रेम सल्फ़ ३० ( Nat sulph-30) का कार्य p-dimethylaminoazobenzene (p-DAB) and phenobarbital (PB)  रसायन खिलाकर लिवर कैन्सर से पीडित हुये  चूहों पर देखना था  ।

इसके लिये चूहों को सात उप समूहों मे विभाजित किया गया ।

१. सामान्य अनुपचारित;

२. सामान्य + एल्कोहल उपचारित

३.  p-DAB (0.06%) + PB (0.05%);

४. p-DAB + PB + एल्कोहल उपचारित

५. p-DAB + PB + Nat sulph-30

६. p-DAB + PB + Car-200

७. p-DAB + PB + Nat sulph-30+Car-200

इन चूहों को क्रमश: ३०. ६०. ९०.और १२० दिनों के अंतराल पर मारकर उनके cytogenetical end-points जैसे chromosome aberrations, micronuclei, mitotic index , sperm head anomaly और cytotoxicity का विशलेषण  किया गया । इसके अलावा, इलेक्ट्रॉन सूक्ष्म अध्ययन और मैट्रिक्स metalloproteinases के लिए जेलाटीन zymography (एम एम पी) जिगर में 90 और 120 पर किये गये ।

परिणाम :

परिणाम दिखाते हैं कि सिर्फ़ नैट्रेम सल्फ़ ३० ( Nat sulph-30) पर  रखे गये कैन्सर से पीडित चूहों और कारसीनोसिन २०० को नैट्र्म सल्फ़ के साथ संयोजित करके चूहों मे लीवर की कई गांठॊ ( liver tumours ) मे व्यापक सुधार दिखाई दिया और  कई क्षेत्रों मे सकारात्मक और संरचनात्मक परिवर्तन दिखाई दिये जैसे MMPs expression, genotoxic parameters, lipid peroxidation, y-glutamyl transferase, lactate dehydrogenase, blood glucose, bilirubin, creatinine, urea and increased GSH, glucose-6-phosphate dehydrogenase, superoxide dismutase, catalase, glutathione reductase activities and hemoglobin, cholesterol, and albumin levels.मे व्यापक सुधार दिखाई दिया ।

नैट्रेम सल्फ़ ३० (Nat sulph-30 )के साथ carcinocin 200 (कारसीनोसिन २००) को देने से genotoxicity , cytotoxicity hepatotoxicity के खिलाफ अतिरिक्त लाभ दिखाई दिये |

 

 Homeopathic drugs Natrum sulphuricum and Carcinosin prevent azo dye-induced hepatocarcinogenesis in mice. Nandini Bhattacharjee, Pathikrit Banerjee and Anisur Rahman Khuda-Bukhshh. Indian Journal of Biochemistry & Biophysics’, Vol-46, August 2009, PP. 307-318.

The study was undertaken to examine whether Carcinosin-200 (Car-200) could provide additional ameliorative effect, if used intermittently with Natrum sulphuricum-30 (Nat sulph-30) against hepatocarcinogenesis induced by chronic feeding of p-dimethylaminoazobenzene (p-DAB) and phenobarbital (PB) in mice (Mus musculus).

Mice were randomly divided into seven sub-groups:

(i) normal untreated;

(ii) normal+succussed alcohol;

(iii) p-DAB (0.06%) + PB (0.05%);

(iv) p-DAB + PB +succussed alcohol

(v) p-DAB + PB + Nat sulph-30,

(vi) p-DAB + PB + Car-200, and

(vii) p-DAB + PB + Nat sulph-30+Car-200.

They were sacrificed at 30, 60, 90 and 120 days for assessment of genotoxicity through cytogenetical end-points like chromosome aberrations, micronuclei, mitotic index and sperm head anomaly and cytotoxicity through assay of widely accepted biomarkers and pathophysiological parameters.

Additionally, electron microscopic studies and gelatin zymography for matrix metalloproteinases (MMPs) were conducted in liver at 90 and 120 days.

Results showed that administration of Nat sulph-30 alone and in combination with Car-200 reduced the liver tumors with positive ultra- structural changes and in MMPs expression, genotoxic parameters, lipid peroxidation, y-glutamyl transferase, lactate dehydrogenase, blood glucose, bilirubin, creatinine, urea and increased GSH, glucose-6-phosphate dehydrogenase, superoxide dismutase, catalase, glutathione reductase activities and hemoglobin, cholesterol, and albumin levels.

Thus intermittent use of Car-200 along with Nat sulph-30 yielded additional benefit against genotoxicity, cytotoxicity hepatotoxicity and oxidative stress induced by the carcinogens during hepatocarcinogenesis.

Is homeopathy compatible with cancer therapy?

Source : CAM Report

This Cochrane review evaluated homeopathic medicines used to prevent or treat side effects of cancer treatments.

First, the details.

• 8 studies in 664 participants were selected for review.
  • 3 studied adverse effects of radiotherapy
  • 3 studied adverse effects of chemotherapy
  • 2 studied menopausal symptoms associated with breast cancer treatment

And, the results.

• 2 studies in 254 patients reported superiority of topical calendula (aka marigold) over trolamine (a topical non-steroidal drug) to prevent radiotherapy-induced dermatitis.
• A study of 32 patients demonstrated superiority of Traumeel S over placebo as a mouthwash for chemotherapy-induced stomatitis (inflammation of the mouth).
  • Traumeel is a homeopathic anti-inflammatory and analgesic preparation containing 12 botanicals and 2 minerals.
• 2 other studies reported positive results, although the risk of bias was unclear.
• 4 studies reported negative results.

The bottom line?
Yes, there’s some supporting evidence for calendula and Traumeel S, concluded the authors. However, “there is no convincing evidence for the efficacy of homeopathic medicines for other adverse effects of cancer treatments.”

Regarding radiotherapy-induced dermatitis: Researchers from Princess Margaret Hospital, in Ontario, Canada conducted a review to evaluate prevention and management of acute skin reactions related to radiation therapy. They concluded, “Skin washing… should be permitted in patients receiving radiation therapy to prevent acute skin reaction.”

They also concluded, “There is insufficient evidence to support or refute specific topical or oral agents [including calendula] for the prevention or management of acute skin reaction.”

Regarding chemotherapy-induced stomatitis: NHS Trust has published an algorithm to guide management of stomatitis and mucositis for oncology patients receiving chemotherapy.