Research showing homeopathic medicines produce apoptosis in cancer cells

New study adds to the growing body of research showing homeopathic medicines produce apoptosis in cancer cells

In a new study published in The International Journal of Oncology the findings of a preclinical study clearly delineated the molecular mechanism underlying the apoptogenic effect of the non-toxic homeopathic remedy, sulphur, in non-small cell lung carcinoma cells.

Sulphur alters NF ?B-p300 cross-talk in favour of p53-p300 to induce apoptosis in non-small cell lung carcinoma

http://www.spandidos-publications.com/ijo/47/2/573

In the search for new medicines devoid of the toxic side effects associated with conventional chemotherapy, this study adds to the growing body of knowledge (1) (2) (3)  that outlines the potential of homeopathic medicines to have a role in the treatment of patients suffering various forms of carcinoma.

1. Contribution of the ROS-p53 feedback loop in thuja-induced apoptosis of mammary epithelial carcinoma cells
   http://www.spandidos-publications.com/or/31/4/1589
2. Cytotoxic effects of ultra-diluted remedies on breast cancer cells
   http://www.spandidos-publications.com/ijo/36/2/395
3. DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells
   http://www.sciencedirect.com/science/article/pii/S147549161400099X

source : http://www.homeobook.com/research-showing-homeopathic-medicines-produce-apoptosis-in-cancer-cells/

कैन्सर कोशिकाओं पर होम्योपैथी दवाओं के प्रयोग – Homeopathic drugs Natrum sulphuricum and Carcinosin prevent azo dye-induced hepatocarcinogenesis in mice

पिछ्ले दिनों ICRH पर कैन्सर कोशिकाओं पर होम्योपैथिक दवाओं के प्रयोगों पर दो रिसर्च पेपर पब्लिश हुये । कल्याणी यूनिवर्सिटी के श्री अनीसुर रहमान खुदाबख्श और उनके सहयोगियों का  ’Homeopathic drugs Natrum sulphuricum and Carcinosin prevent azo dye-induced hepatocarcinogenesis in mice’ और आमला कैन्सर रिसर्च सेन्टर , केरल के रामदास कुटन , हरीकुमार और अन्य का शोध पत्र , ’ Inhibition of Chemically Induced Carcinogenesis by Drugs Used in Homeopathic Medicine ’ । इन दोनों शोध पत्रों मे ’ Inhibition of Chemically Induced Carcinogenesis by Drugs Used in Homeopathic Medicine ’ मे लगभग वही तथ्यों को दोहराया गया जो इसके पहले प्रशान्त बैनर्जी कैन्सर रिसर्च फ़ाउन्डेशन ने अपने कई शोध पत्रों मे रुटा और उअसके पर्योगों पर दिये  थे , देखें विस्तृत वर्णन के लिये यहाँ ।

लेकिन बात करते हैं अनीसुर रहमान खुदाबख्श के शोध पत्र की । यह पेपर लीक से हट्कर था । प्रथम तो इसमे होम्योपैथी के मूलभूत सिद्धातों (  एकमैव औषधि- single medicine ) को नजरांदाज किया गया और दूसरा औषधि के परीक्षण के लिये चूहों का सहारा लिया गया । तो सबसे पहले विवरण :

विवरण :

इस शोध  का मकसद होम्योपैथिक औषधि कारसीनोसिन २०० ( carcinocin 200 ) और नैट्रेम सल्फ़ ३० ( Nat sulph-30) का कार्य p-dimethylaminoazobenzene (p-DAB) and phenobarbital (PB)  रसायन खिलाकर लिवर कैन्सर से पीडित हुये  चूहों पर देखना था  ।

इसके लिये चूहों को सात उप समूहों मे विभाजित किया गया ।

१. सामान्य अनुपचारित;

२. सामान्य + एल्कोहल उपचारित

३.  p-DAB (0.06%) + PB (0.05%);

४. p-DAB + PB + एल्कोहल उपचारित

५. p-DAB + PB + Nat sulph-30

६. p-DAB + PB + Car-200

७. p-DAB + PB + Nat sulph-30+Car-200

इन चूहों को क्रमश: ३०. ६०. ९०.और १२० दिनों के अंतराल पर मारकर उनके cytogenetical end-points जैसे chromosome aberrations, micronuclei, mitotic index , sperm head anomaly और cytotoxicity का विशलेषण  किया गया । इसके अलावा, इलेक्ट्रॉन सूक्ष्म अध्ययन और मैट्रिक्स metalloproteinases के लिए जेलाटीन zymography (एम एम पी) जिगर में 90 और 120 पर किये गये ।

परिणाम :

परिणाम दिखाते हैं कि सिर्फ़ नैट्रेम सल्फ़ ३० ( Nat sulph-30) पर  रखे गये कैन्सर से पीडित चूहों और कारसीनोसिन २०० को नैट्र्म सल्फ़ के साथ संयोजित करके चूहों मे लीवर की कई गांठॊ ( liver tumours ) मे व्यापक सुधार दिखाई दिया और  कई क्षेत्रों मे सकारात्मक और संरचनात्मक परिवर्तन दिखाई दिये जैसे MMPs expression, genotoxic parameters, lipid peroxidation, y-glutamyl transferase, lactate dehydrogenase, blood glucose, bilirubin, creatinine, urea and increased GSH, glucose-6-phosphate dehydrogenase, superoxide dismutase, catalase, glutathione reductase activities and hemoglobin, cholesterol, and albumin levels.मे व्यापक सुधार दिखाई दिया ।

नैट्रेम सल्फ़ ३० (Nat sulph-30 )के साथ carcinocin 200 (कारसीनोसिन २००) को देने से genotoxicity , cytotoxicity hepatotoxicity के खिलाफ अतिरिक्त लाभ दिखाई दिये |

 

 Homeopathic drugs Natrum sulphuricum and Carcinosin prevent azo dye-induced hepatocarcinogenesis in mice. Nandini Bhattacharjee, Pathikrit Banerjee and Anisur Rahman Khuda-Bukhshh. Indian Journal of Biochemistry & Biophysics’, Vol-46, August 2009, PP. 307-318.

The study was undertaken to examine whether Carcinosin-200 (Car-200) could provide additional ameliorative effect, if used intermittently with Natrum sulphuricum-30 (Nat sulph-30) against hepatocarcinogenesis induced by chronic feeding of p-dimethylaminoazobenzene (p-DAB) and phenobarbital (PB) in mice (Mus musculus).

Mice were randomly divided into seven sub-groups:

(i) normal untreated;

(ii) normal+succussed alcohol;

(iii) p-DAB (0.06%) + PB (0.05%);

(iv) p-DAB + PB +succussed alcohol

(v) p-DAB + PB + Nat sulph-30,

(vi) p-DAB + PB + Car-200, and

(vii) p-DAB + PB + Nat sulph-30+Car-200.

They were sacrificed at 30, 60, 90 and 120 days for assessment of genotoxicity through cytogenetical end-points like chromosome aberrations, micronuclei, mitotic index and sperm head anomaly and cytotoxicity through assay of widely accepted biomarkers and pathophysiological parameters.

Additionally, electron microscopic studies and gelatin zymography for matrix metalloproteinases (MMPs) were conducted in liver at 90 and 120 days.

Results showed that administration of Nat sulph-30 alone and in combination with Car-200 reduced the liver tumors with positive ultra- structural changes and in MMPs expression, genotoxic parameters, lipid peroxidation, y-glutamyl transferase, lactate dehydrogenase, blood glucose, bilirubin, creatinine, urea and increased GSH, glucose-6-phosphate dehydrogenase, superoxide dismutase, catalase, glutathione reductase activities and hemoglobin, cholesterol, and albumin levels.

Thus intermittent use of Car-200 along with Nat sulph-30 yielded additional benefit against genotoxicity, cytotoxicity hepatotoxicity and oxidative stress induced by the carcinogens during hepatocarcinogenesis.

Inhibition of Chemically Induced Carcinogenesis by Drugs Used in Homeopathic Medicine* K.B. Hari Kumar, E.S. Sunila, Girija Kuttan, K.C. Preethi, C. Nimita Venugopal, Ramadasan Kuttan

Source :  INDIAN JOURNAL OF RESEARCH IN HOMEOPATHY

Homeopathy is considered as one modality for cancer therapy. However, there are only very few clinical reports on the activity of the drugs, as well as in experimental animals. Presently we have evaluated the inhibitory effects of potentized homeopathic preparations against N’- nitrosodiethylamine (NDEA) induced hepatocellular carcinoma in rats as well as 3- methylcholanthrene-induced sarcomas in mice. We have used Ruta, Hydrastis, Lycopodium and Thuja, which are commonly employed in homeopathy for treating cancer. Administration of NDEA in rats resulted in tumor induction in the liver and elevated marker enzymes such as gamma-glutamyl transpeptidase, glutamate pyruvate transaminase, glutamate oxaloacetate transaminase and alkaline phosphatase in the serum and in liver. Concomitant administration of homeopathic drugs retarded the tumor growth and significantly reduced the elevated marker enzymes level as revealed by morphological, biochemical and histopathological evaluation. Out of the four drugs studied, Ruta 200c showed maximum inhibition of liver tumor development. Ruta 200c and phosphorus 1M were found to reduce the incidence of 3-methylcholanthreneinduced sarcomas and also increase the life span of mice harboring the tumours. These studies demonstrate that homeopathic drugs, at ultra low doses, may be able to decrease tumor induction by carcinogen administration. At present we do not know the mechanisms of action of these drugs useful against carcinogenesis.
Keywords: N’-Nitrosodiethylamine; hepatocellular carcinoma; sarcoma; homeopathy; Ruta; Thuja; Hydrastis; Lycopodium; phosphorus

‘The article was originally published in the journal Asian Pacific Journal & Cancer Prevention.’ Vol. 8.2007.
republished in Indian Journal of Research in Homoeopathy Vol. 3. No. 3. July-September 2009
Reprint with the consent of the author and the publisher. "Address for CorrespondenceAmala Cancer Research Centre. Amala Nagar. Thrissur, Kerala State, India. 680555. E-Mail: amalaresearch@rediffmail.com

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